RNA Details
Disease Name
pulmonary arterial hypertension
Tissue
lung
RNA Symbol
Tp73
RNA ID
RNA Type
mRNA
Alteration Pattern
dysregulation
Species
rattus norvegicus
Detection Methods
Immunohistochemistry; qRT-PCR; Immunofluorescence etc.
Target
NA
Pathway
NA
PubMed ID
Title
Hypoxia potentiates microRNA-mediated gene silencing through posttranslational modification of Argonaute2
Year
2011
Function
"Hypoxia increases type I collagen prolyl-4-hydroxylase [C-P4H(I)], which leads to prolyl-hydroxylation and accumulation of Argonaute2 (Ago2). Hydroxylation of Ago2 is required for the association of Ago2 with heat shock protein 90 (Hsp90), which is necessary for the loading of microRNAs (miRNAs) into the RISC, and translocation to stress granules (SGs). We demonstrate that hydroxylation of Ago2 increases the level of miRNAs and increases the endonuclease activity of Ago2. In summary, this study identifies hypoxia as a mediator of the miRNA-dependent gene silencing pathway through posttranslational modification of Ago2, which might be responsible for pulmonary artery hypertension (PAH)."